I created a few parts last night, cshut everything down and cleaned up and went to bed. I wake up today and I go to make a few other parts and when I run Mach3, it tells me that an External E-Stop was requested and it won't let me run the machine. I'm using the G540 controller and everything seems to be working fine otherwise but it won't move the machine. Any ideas?

Sounds like you opened up Mach3 using a Different Profile....

Did you try the reset button...?

I tried everything...ends up that I had to remove the profile for the gecko driver and put a new one fro mtheir website in...worked like a champ after that.

Glad to see your back up and Running.....